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Imaging


Digital images of the stained sections and autoradiograms are obtained using a Northern Lights Illuminator light box (Imaging Research) and a Sony XC-77 CCD camera that is attached to an enlarger stand and is fitted with a lens stack to allow focusing from a distance of about 5 cm. The camera is plugged into a frame grabber card (LG-3 Scientific PCI from Scion Corporation) in a Macintosh G3 computer running NIH IMAGE 1.62 software. The images of the largest bulb sections fill the window of the application under these conditions. The focus of the section is adjusted by changing the height of the camera. Different light intensities are used for the cresyl violet-stained sections and the autoradiograms, but the intensity is held constant for both the autoradiographic images and the standards that are on the same film. 

 
 
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UC Irvine HBP NIDCD NIMH
 
This Human Brain Project/Neuroinformatics project is funded by the National Institute on Deafness and Other Communication Disorders and the National Institute of Mental Health