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| Imaging |
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Digital images of the stained sections and
autoradiograms are obtained using a Northern Lights
Illuminator light box (Imaging Research) and a Sony
XC-77 CCD camera that is attached to an enlarger stand
and is fitted with a lens stack to allow focusing from a
distance of about 5 cm. The camera is plugged into a
frame grabber card (LG-3 Scientific PCI from Scion
Corporation) in a Macintosh G3 computer running NIH
IMAGE 1.62 software. The images of the largest bulb
sections fill the window of the application under these
conditions. The focus of the section is adjusted by
changing the height of the camera. Different light
intensities are used for the cresyl violet-stained
sections and the autoradiograms, but the intensity is
held constant for both the autoradiographic images and
the standards that are on the same film.
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