1981 B.A.
Biochemistry University
of Kansas
Lawrence,
KS
1986 Ph.D. Biological
Sciences University
of California, Irvine
Irvine,
CA
RESEARCH TRAINING & EMPLOYMENT
8/80-8/81: Undergraduate
study and Research Assistant in the laboratory of Dr. Elias Michaelis, Dept. of
Biochemistry, University of Kansas.
9/81-4/86: Graduate
study in the laboratory of Dr. Dana Aswad, Dept. of Psychobiology, University
of California, Irvine.
5/86-1/90: Postgraduate
Researcher in the laboratory of Dr. Dana Aswad.
1/90-7/04: Assistant
Researcher in the Dept. of Neurobiology and Behavior, University of California,
Irvine.
7/04-present: Associate Project Scientist,
Dept. of Neurobiology and Behavior, University of California, Irvine.
AWARDS & HONORS
Graduation with Distinction and Honors in
Biochemistry, University of Kansas, 1981
Phi Beta Kappa, 1981
Regents' Fellowship, University of
California, 1981
PHS National Research Service Award, 1984
Edward A. Steinhaus Graduate Student
Teaching Award, School of Biological Sciences, University of California,
Irvine, 1985
Regents' Dissertation Fellowship,
University of California, 1986
Takasago Award for Research in Olfaction,
2003
PROFESSIONAL
SOCIETIES
Society
for Neuroscience
Association
for Chemoreception Sciences
EDITORIAL BOARD
Chemosensory Perception
DISSERTATION
Johnson,
B.A. (1986) Modification of L-isoaspartate by protein carboxyl
methyltransferase, University of California, Irvine.
PUBLICATIONS
1. Michaelis,
E., Chittenden, W., Johnson, B., Galton, N., and Decedue, C. (1984)
Purification, biochemical characterization, binding activity, and selectivity
of a glutamate binding protein from bovine brain. J. Neurochem. 42, 397-406.
2. Johnson,
B.A., and Aswad, D.W. (1985) Identification and topography of substrates for
protein carboxyl methyltransferase in synaptic membrane and myelin-enriched fractions
of bovine and rat brain. J. Neurochem. 45,
1119-1127.
3. Johnson,
B.A., Freitag, N.E., and Aswad, D.W. (1985) Protein carboxyl methyltransferase
selectively modifies an atypical form of calmodulin. Evidence for methylation at deamidated asparagine residues. J.
Biol. Chem. 260, 10913-10916.
4. Johnson,
B.A., and Aswad, D.W. (1985) Enzymatic protein carboxyl methylation at
physiological pH: Cyclic imide
formation explains rapid methyl turnover. Biochemistry 24, 2581-2586.
5. Aswad,
D.W., Johnson, B.A., and Glass, D.B. (1987) Modification of synthetic peptides
related to lactate dehydrogenase (231-242) by protein carboxyl
methyltransferase and tyrosine protein kinase: Effect of introducing an isopeptide bond between Asp-235 and
Ser-236. Biochemistry
26, 675-681.
6. Aswad,
D.W., and Johnson, B.A. (1987) The unusual substrate specificity of eucaryotic
protein carboxyl methyltransferases. Trends Biochem. Sci. 12, 155-158.
7. Johnson,
B.A., Murray, E.D., Jr., Clarke, S., Glass, D.B., and Aswad, D.W. (1987)
Protein carboxyl methyltransferase facilitates conversion of atypical
L-isoaspartyl peptides to normal L-aspartyl peptides. J. Biol. Chem. 262, 5622-5629.
8. Johnson,
B.A., Langmack, E.L., and Aswad, D.W. (1987) Partial repair of
deamidation-damaged calmodulin by protein carboxyl methyltransferase. J.
Biol. Chem. 262, 12283-12287.
9. Aswad,
D.W., Johnson, B.A., Langmack, E.L., and Shirokawa, J.M. (1988) Modification of
isoaspartyl peptides and proteins by protein carboxyl methyltransferase from
bovine brain. In: Post-translational Modifications of Proteins and Ageing (Zappia, V., Galletti, P., Porta, R.,
and Wold, F., eds.) pp. 247-259, Plenum Publishing Corporation, New York, NY.
10. Johnson,
B.A., Shirokawa, J.M., and Aswad, D.W. (1989) Deamidation of calmodulin at
neutral and alkaline pH: Quantitative relationships between ammonia loss and
the susceptibility of calmodulin to modification by protein carboxyl
methyltransferase. Arch. Biochem. Biophys. 268,
276-286.
11. Johnson,
B.A., Shirokawa, J.M., Hancock, W.S., Spellman, M.W., Basa L.J., and Aswad,
D.W. (1989) Formation of isoaspartate at two distinct sites during in vitro
aging of human growth hormone. J. Biol. Chem. 264, 14262-14271.
12. Johnson,
B.A., and Aswad, D.W. (1990) Identities, origins, and metabolic fates of the
substrates for eukaryotic protein carboxyl methyltransferases. In: Protein
Methylation (Paik,
W.-K., and Kim, S., eds.) pp. 195-210, CRC Press, Boca Raton, FL.
13. Johnson,
B.A., and Aswad, D.W. (1990) Fragmentation of isoaspartyl peptides and proteins
by carboxypeptidase Y: Release of isoaspartyl dipeptides as a result of
internal and external cleavage. Biochemistry 29, 4373-4380.
14. Johnson,
B.A., Shirokawa, J.M., Geddes, J.W., Choi, B.H., Kim, R.C., and Aswad, D.W.
(1991) Protein L-isoaspartyl methyltransferase in postmortem brains of aged
humans. Neurobiol. Aging
12, 19-24.
15. Johnson,
B.A., and Aswad, D.W. (1991) Optimal conditions for the use of protein L‑isoaspartyl
methyltransferase in assessing the isoaspartate content of peptides and
proteins. Anal. Biochem.
192, 384-391.
16. Johnson,
B.A., Ngo, S.Q., and Aswad, D.W. (1991) Widespread phylogenetic distribution of
a protein methyltransferase that modifies L-isoaspartyl residues. Biochem.
Int. 24, 841-847.
17. Najbauer,
J., Johnson, B.A., and Aswad, D.W. (1991) Amplification and detection of
substrates for protein carboxyl methyltransferases in PC12 cells. Anal.
Biochem. 197, 412-420.
18. Najbauer,
J., Johnson, B.A., and Aswad, D.W. (1992) Analysis of stable protein
methylation in cultured cells. Arch. Biochem. Biophys. 293, 85-92.
19. Potter,
S.M., Johnson, B.A., Henschen, A., Aswad, D.W., and Guzzetta, A.W. (1992) The
type II isoform of bovine brain protein L-isoaspartyl methyltransferase has an
endoplasmic reticulum retention signal (...RDEL) at its C-terminus. Biochemistry 31, 6339-6347.
20. Johnson,
B.A., and Aswad, D.W. (1993) Kinetic properties of bovine brain protein
L-isoaspartyl methyltransferase determined using a synthetic isoaspartyl
peptide substrate. Neurochem. Res.
18, 87-94.
21. Johnson,
B.A., Najbauer, J., and Aswad, D.W. (1993) Accumulation of substrates for
protein L-isoaspartyl methyltransferase in adenosine dialdehyde-treated PC12
cells. J. Biol. Chem.
268, 6174-6181.
22. Najbauer,
J., Johnson, B.A., Young, A.L., and Aswad, D.W. (1993) Peptides with sequences
similar to glycine, arginine-rich motifs in proteins interacting with RNA are
efficiently recognized by methyltransferase(s) modifying arginine in numerous
proteins. J. Biol. Chem.
268, 10501-10509.
23. Johnson,
B.A., and Aswad, D.W. (1995) Deamidation and isoaspartate formation during in
vitro aging of purified proteins.
In: Deamidation and Isoaspartate Formation in Peptides and Proteins (Aswad, D.W., ed.) pp. 91-113, CRC
Press, Boca Raton, FL.
24. Johnson,
B.A., Woo, C.C., Duong, H., Nguyen, V., and Leon, M. (1995) A learned odor
evokes an enhanced Fos-like glomerular response in the olfactory bulb of young
rats. Brain Res. 699, 192-200.
25. Johnson,
B.A., Woo, C.C., Ninomiya-Tsuboi, K., and Leon, M. (1996) Synaptophysin-like
immunoreactivity in the rat olfactory bulb during development and after
restricted early olfactory experience. Dev. Brain Res. 92, 24-30.
26. Johnson,
B.A., and Leon, M. (1996) Spatial distribution of [14C]2-deoxyglucose
uptake in the glomerular layer of the rat olfactory bulb following early odor
preference learning. J. Comp.
Neurol. 376, 557-566.
27. Johnson,
B.A., Woo, C.C., and Leon, M. (1998) Spatial coding of odorant features in the
glomerular layer of the rat olfactory bulb. J. Comp. Neurol. 393, 457-471.
28. Johnson, B., and Leon, M. (1999) The role
of early experience in olfactory bulb cell survival. In: The Biology of Early Influences (Hyson, R.L., and Johnson, F., eds.) pp.
73-85, Kluwer Academic/Plenum Press, New York.
29.
Leon, M., and Johnson, B. (1999) Experiential basis for chemical coding. Dev. Neuropsychol. 16, 321-323.
30.
Johnson, B.A., Woo, C.C., Hingco, E.E.,
Pham, K.L., and Leon, M. (1999) Multidimensional, chemotopic responses to
n-aliphatic acid odorants in the rat olfactory bulb. J. Comp. Neurol. 409, 529-548.
31. Johnson, B.A., and Leon, M. (2000) Modular
representations of odorants in the rat olfactory bulb: the effects of stimulus
concentration. J. Comp. Neurol.
422, 496-509.
32. Johnson, B.A., and Leon, M. (2000)
Odorant molecular length: one aspect of the olfactory code. J. Comp. Neurol. 426, 330-338.
33. Johnson, B.A., and Leon, M. (2001)
Spatial coding in the olfactory bulb: the role of early experience. In: Developmental Psychobiology, Developmental Neurobiology
and Behavioral Ecology: Mechanisms
and Early Principals. (Blass,
E.M., ed.) pp. 53-80, Kluwer Academic/Plenum
Publishers, New York.
34. Johnson, B.A., and Leon, M. (2001)
Spatial representations of odorant chemistry in the main olfactory bulb of the
rat. In: Chemical Signals in Vertebrates IX (Marchlewska-Koj, A., Lepri, J.J., and
Muller-Schwarze, D, eds.) pp. 85-91, Kluwer Academic/Plenum
Publishers, New York.
35. Linster, C., Johnson, B.A., Yue, E.,
Morse, A., Xu, Z., Hingco, E.E., Choi, Y., Choi, M., Messiha, A., and Leon, M.
(2001) Perceptual correlates of neural representations evoked by odorant enantiomers.
J. Neurosci. 21, 9837-9843.
36. Johnson, B.A., Ho, S.L., Xu, Z., Yihan,
J.S., Yip, S., Hingco, E.E., and Leon, M. (2002) Functional mapping of the rat
olfactory bulb using diverse odorants reveals modular responses to functional
groups and hydrocarbon structural features. J. Comp. Neurol. 449, 180-194.
37. Linster, C., Johnson, B.A., Morse, A.,
Yue, E., and Leon, M. (2002) Spontaneous versus
reinforced olfactory discriminations. J. Neurosci. 22, 6842-6845.
38. Ferrari, C.C., Johnson, B.A., Leon, M.,
and Pixley, S.K. (2003) Spatiotemporal distribution of the insulin-like growth
factor receptor in the rat olfactory bulb. Neurochem. Res. 28, 29-43.
39. Leon, M., and Johnson, B.A. (2003)
Olfactory coding in the mammalian olfactory bulb. Brain Res. Rev. 42, 23-32.
40. Johnson, B.A., Farahbod, H., Xu, Z.,
Saber, S., and Leon, M. (2004) Local and global chemotopic organization:
general features of the glomerular representations of aliphatic odorants
differing in carbon number. J. Comp. Neurol. 480, 234-249.
41. Johnson, B.A., Farahbod, H., Saber, S.,
and Leon, M. (2005) Effects of functional group position on spatial
representations of aliphatic odorants in the rat olfactory bulb. J. Comp.
Neurol. 483, 192-204.
42. Johnson, B.A., Farahbod, H., and Leon, M.
(2005) Interactions between odorant functional group and hydrocarbon structure
influence activity in glomerular response modules in the rat olfactory bulb.
J. Comp. Neurol. 483, 205-216.
43. Farahbod, H., Johnson, B.A., Minami, S.S., and Leon, M. (2006) Chemotopic representations of aromatic odorants in the rat olfactory bulb.
J. Comp. Neurol. 496, 350-366
44. Ho, S.L., Johnson, B.A., and Leon, M.
(2006) Long hydrocarbon chains serve as unique molecular features recognized by
ventral glomeruli of the rat olfactory bulb. J. Comp. Neurol. 498, 16-30.
45. Johnson, B.A., Xu, Z., Kwok, J., Pancoast, P., Ong, J., and Leon, M. (2006) Differential specificity in the glomerular response profiles for alicyclic, bicyclic and heterocyclic odorants. J. Comp. Neurol. 499, 1-16.
46. Leon, M., and Johnson, B. (2006) Functional units in the olfactory system. Proc. Natl. Acad. Sci. USA 103, 14985-14986.
47. Ho, S.L., Johnson, B.A., Chen, A.L., and Leon, M. (2006) Differential responses to branched and unsaturated aliphatic hydrocarbons in the rat olfactory system. J. Comp. Neurol. 499, 519-532.
48. Youngentob, S.L., Johnson, B.A., Leon, M., Sheehe, P.R., and Kent, P.F. (2006) Predicting odorant quality perceptions from multidimensional scaling of olfactory bulb glomerular activity patterns. Behav. Neurosci. 120, 1337-1345.
49. Woo, C.W., Hingco, E.E., Johnson, B.A., and Leon, M. (2007) Broad activation of the glomerular layer enhances subsequent olfactory responses. Chem. Senses 32, 51-55.
50. Johnson, B.A., Ong, J., Lee, K., Ho, S.L., Arguello, S., and Leon, M. (2007) Effects of double and triple bonds on the spatial representations of odorants in the rat olfactory bulb. J. Comp. Neurol. 500, 720-733.
51. Cleland, T.A., Johnson, B.A., Leon, M., and Linster, C. (2007) Relational representation in the olfactory system. Proc. Natl. Acad. Sci. USA 104, 1953-1958.
52. Johnson, B.A., Arguello, S., and Leon, M. (2007) Odorants with multiple oxygen-containing functional groups and other odorants with high water solubility preferentially activate posterior olfactory bulb glomeruli. J. Comp. Neurol. 502, 468-482.
53. Johnson, B.A., and Leon, M. (2007) Chemotopic odorant coding in a mammalian olfactory system. J. Comp. Neurol. 503, 1-34.
54. Johnson, B.A., and Leon, M. (2007)
Understanding olfactory coding via an analysis of odorant-evoked glomerular response maps. In: The Senses Ð A Comprehensive Reference. Elsevier, London, in press.
55. Johnson, B.A., and Leon, M. (2007)
Glomerular map. In: Encyclopedic Reference of Neuroscience. Springer-Verlag, Heidelberg, in press.